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During the cycle, consistency matters more than any particular daily timing

The animals were treated daily during five weeks with L-NAME (10 mg/kg b.w.), L-arginine (100 mg/kg b.w.), NaBPC157 (10 g/kg b.w.) and their combinations (L-NAME + NaBPC157, L-NAME + L-arginine) intraperitoneally

Extension: We fully flex the MCP joints and measure the angle as extending the MCP joint to the maximum
