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For long-term storage, freezing at -20C helps keep it pure and stable
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After the treatment mentioned above, the cells were washed twice with PBS and then incubated with 2M calcein-acetoxymethyI ester (GLPBIO, USA) at 37C for 30 minutes
It has been proposed that antioxidant capacity mediated by the Nrf2 pathway is the key to maintain redox metabolism during aging (97)
