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Polak-Berecka et al., 2013), carotenoids (Kim et al., 2019), ferulic acid (Bhathena et al., 2008), or histamine (Azuma et al., 2001)
After centrifugation and washing 35 times with wash buffer (20 mM Tris-HCl, pH 7.5, 300 mM NaCl, 0.5% Triton X-100, 1 mM EDTA, 1 mM DTT), the protein-bound beads were loaded onto an SDS-PAGE gel for western blot analysis using antibodies against 6 His and GST
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