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qPCR (20 L) was carried out by using 2 Ultra SYBR Mixture (CWbio, China) in 12 8 well plates in a CFX Connect Real-Time System (Bio-Rad), containing 10 L of SYBR Mixture, 2 L cDNA, 1.6 L of qPCR primers (4 M), and 7.4 L nuclease-free water

Morphologically, light and electron microscopic examinations of irradiated testes have revealed marked disorganization, desquamation, and vacuolization of the germ cells of rats seminiferous tubules, as well as formation of multinucleated giant cells in the germinal epithelium following spermatogenesis arrest, compared to the unirradiated testes

These side-effects are possible, but do not always occur