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Pierce BCA protein assay kit was applied to measure the total protein concentration of cell lysates

Although our findings primarily focus on the anti-inflammatory impact of Irisin on microglia and dopaminergic neurons, we recognize the potential significance of astrocyte modulation in Irisins protective mechanisms

Foresta C, Flohe L, Garolla A, Roveri A, Ursini F, Maiorino M

Interestingly, in traumatic brain injury, GPx1 deficiency was associated with decreased mitochondrial bioenergetic capacity compared to wildtype mice, whereas transgenic GPx1 mice had no decrease in mitochondrial function [114] GPx1 deficiency also enhanced apoptosis and inflammation in a cold-induced brain injury model [115]