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In brief, cells were washed once with PBS and lysed by the addition of luciferase lysis buffer (1% Triton X-100, 25 mM Glycyl-Glycin (pH 7.8), 15 mM MgSO 4 , 4 mM EGTA, 1 mM DTT)
Also authors showed that PLC deletion contributed to the lack of transition from low-grade adenoma to high-grade sarcoma
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