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Immunofluorescence The aorta (5 microns thick) was subjected to antigen retrieval by heating it in 10 mM citrate buffer (pH 6.0) at 95 C for 10 min after decalcification and hydration

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The article "The Many Faces of Cobalamin (B12) Deficiency" is excellent for this usage because it is written by physician/researchers, and is quite an accurate and complete complete summary of issues pertaining to B12 deficiency (a miracle in itself), and as such illustrates the seriousness of the disorder in a way that is hard to dismiss and includes description of appropriate treatment for the two general populations of patients with B12 deficiency