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Description
The dried bacterial pellet was solubilized in 20 ml of B-PER (78243, Thermo Fisher Scientific), supplemented with protease inhibitors (1 g ml 1 of leupeptin, 1 g ml 1 of AEBSF HCl and 1 l of benzonase) and 5 mM imidazole and then transferred to a 50-ml centrifuge tube

The LCA model identified three similar longitudinal exposure patterns

As per this report, there has been substantial reductions in body weight over 48 weeks in adults with obesity
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