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When the exosome sample are introduced into the system, the fluorescently labeled short sequence is released into the solution by competitive binding of CD63 protein

Peptides were separated on a 75m (inner diameter) microcapillary column packed with ~0.5 cm of Magic C4 resin (5m, 100 , Michrom Bioresources) followed by ~35 cm of GP-18 resin (1.8 m, 200 , Sepax) using a 1 hr gradient of 5-21% acetonitrile in 0.125% formic acid at a flow rate of ~500 nL/min

doi: 10.3390/pharmaceutics14102101 172 LpezJAGarcaRMartnMA

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