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The Fourier transform infrared (FTIR) spectra of the samples contained a broad IR absorption peak at 37003000 cm 1 , corresponding to -OH stretching vibrations associated with hydrogen bonding, and at 1650 cm 1 , corresponding to asymmetric stretching vibrations of the carboxyl (COO-) group (Fig

However, the increase in molar ratio is entirely explained by the fact that IGF-I and IGFBP-3 increase with the same number of moles per liter, but with a larger relative increase in IGF-I than IGFBP-3 and with IGF-II molar concentrations being unchanged (112)