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This estimates intracellular fluxes by solving a non-linear least-squares regression problem that minimizes the variance-weighted sum of squared residuals between simulated and measured isotopic distributions of intracellular metabolites, under the assumption of pseudo-steady state (verified in Extended Data Fig

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Rabbit anti-Bax and anticaspase-3 antibodies were from Cell Signaling (Cell Signaling, Danvers, MA, USA), and rabbit anti-Bcl-2, anti-GR, and anti-GPx antibodies were from Abcam (Abcam, Cambridge, MA, UK)