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Description
Persister Cell Derivation and Treatment Persister cells were derived from colorectal cancer cell lines HCT116 (KRAS G13D ) and SW620 (KRAS G12V ) treated with 5-FU and AZ628, for at least 9 days with fresh drug added every 3 days, and then stained with crystal violet or counted using a Countess Automated Cell Counter (Invitrogen, Carlsbad, CA, USA)
NADH with low recovery was detected in NAD+ assay (88%) suggesting substantial interconversion of the spiked compound
Animal experiments All animal studies described were approved by the NYU Langone Medical Center Institutional Animal Care and Use Committee
PubMed CrossRef Stahl SM, Felker A
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