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For the electron flow assays, mitochondria were plated in MAS buffer with 10 mM pyruvate + 2 mM malate + 4 M FCCP followed by 20 M rotenone in port A, 100 mM succinate in port B, 40 M antimycin A in port C and 1 mM TMPD + 100 mM ascorbic acid in port D
The NLCs were also confirmed to express P2X 7 R at increased levels compared to the undifferentiated HL-60 (Fig
For example, hospital mortality was 11% in patients treated with placebo and 3.4% in those treated with NAC (p = 0.005) [179]
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