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DMEM/F12 was used as a control for background absorbance
We further evaluated the effect of GCLC succinylation on its enzymatic activity by measuring GSH synthesis rate in U2OS cells transfected with either Flag-tagged WT GCLC or its K-R and K-E mutants

Epub 2014 Aug 13

Another extensive research paper looked at whether the timing of acetaminophen exposure was relevant
