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Tissue fixation and preparation of paraffin-embedded, frozen, and vibratome-cut sections Brain hemispheres were fixed by immersion in 4% paraformaldehyde in phosphate buffer solution (Nacalai tesque, Kyoto, Japan)

Prepare your device:Select an appropriate needle depth based on experience level and treatment goals

In addition, an FSP1-dependent noncanonical VK cycle has recently been reported to function as a ferroptosis suppressor, and FSP1 efficiently reduced VK to its hydroquinone VKH 2 form, which function as a potent radical-trapping antioxidant to inhibit lipid peroxidation[18]

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