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Accordingly, a Ca 2+ imaging study of the human submucosal plexus showed that under physiological conditions, communication from mast cells to nerves was rarely observed when mast cells were activated by IgE receptor crosslinking, which induces the release of a cocktail of mediators including proteases, histamine, and cytokines [122, 123]

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This timeframe is dictated by USP (United States Pharmacopeia) standards for sterile preparations