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Results: There was a significant difference in time of evaluation, with a decrease in time of evaluation moving from Sub1 to Sub4 (p<0.05)

The Nile Red 2p-FLIM hardware platform reported here can be further improved to reach higher imaging speed by choosing a suitable laser pulse repetition rate and photon counting rate or using a wide-field microscope configuration ( While Nile Red staining has been considered unreliable for identifying lipid structures in live C

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