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An FEI Vitrobot Mark IV (Thermo Fisher Scientific) was used to plunge-freeze the grids into liquid ethane after sample application at 4 C and 100% humidity, with a blot time of 3 s and a blot force of 5

Despite the availability of pharmacological treatments, these interventions fail to prevent disease progression due to their limited ability to penetrate the blood-brain barrier (BBB) and systemic side effects

Discussing expectations with your physician to ensure the best course of action for your needs is essential
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