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Description
The tissue samples were homogenized in PBS (pH 7.4) containing 0.1 mmol/L butylated hydroxytoluene (BHT) (TissueRuptor, Qiagen, United States) and sonicated for 30 s in an ice bath (Ultrasonic bath, Branson, United States)
Ba replied that he would not take instructions from Americans
This combination addresses both local gut tissue repair and overall inflammatory burden
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