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Sequencing, data processing, and analysis parameters The study's primary aim was to determine whether 5A-1MQ treatment in combination with a switch from WD to LD rendered the intestinal microbiome comparable to that of mice continuously maintained on LD, and distinct from that of DIO mice switched to LD but given saline injections or mice maintained continuously on WD

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Second, an in vitro chromosome aberration assay was performed in Chinese Hamster Ovary (CHO) cells treated with AOD9604 (20, 50, 100, 200 g/mL) in the absence or presence of an S-9 metabolic activation system