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The PCR product of N0362 was cloned into the pET100/D-TOPO expression vector (Life Technologies), which encodes a 6xHis-tag at the N-terminus

In GCF concentrations of glutathione (range 0.5-2.5 mM) were reported to be 1000 times greater than that normally detected within extracellular tissue compartments (0.5-5 M in human plasma).[29] The high levels of glutathione within GCF may be the result of increased synthesis by cells of the periodontal tissues, or of active release mechanisms or passive release secondary to protease activity on gingival epithelial cells.[4] Neutrophils and/or junctional/crevicular epithelium (JE) are significant contributors for the high concentrations of glutathione in GCF

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62 13491355 Mandavilli BS, Santos JH and Van Houten B 2002 Mitochondrial DNA repair and aging