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Materials and Methods RAW264 Cells, GSH Depletion, and Treatment RAW264 cells were cultured in Roswell Park Memorial Institute medium 1640 with 2 mM l-glutamine (Sigma), 100 U/ml penicillin, 100 g/ml streptomycin sulfate (Invitrogen/Life Technologies), and 10% heat-inactivated FCS (Sigma)-omplete medium
An illustrative reminder of the skin's structure is shown in the diagram below
The neurons were washed in PBS and then mounted in Mowiol
Beta-aminoisobutyrate is a product formed by the catabolism of thymine, one of the four nucleobases in the nucleic acid of DNA
