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Description
Gastrointestinal disorders and surgeries Some GI-related conditions and surgeries may affect B12 absorption: Crohns disease: A chronic inflammatory disease, Crohns affects the small and large intestine particularly the distal ileum, which is where B12 is absorbed
Beauchamp, C
Nature 486 , 400404 (2012)
Affinity purification performed by gravity using TALON resin (Clontech), anion exchange performed using HiTrapQ FF (GE Healthcare), size-exclusion chromatography performed using Superdex 200 10/30 prep grade (GE Healcare) the later two performed using KTA FPLC platform
