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Successive decarboxylations (by uroporphyrinogen decarboxylase, UROD) remove four acetate side chains to yield coproporphyrinogen III, which re-enters the mitochondria for oxidative decarboxylation to protoporphyrinogen IX by coproporphyrinogen oxidase (CPOX), followed by aromatization to protoporphyrin IX by protoporphyrinogen oxidase (PPOX)

Mast cells are present in the brain and its meninges, including the area postrema, choroid plexus, and thalamic-hypothalamic region [212]

For the analysis of U87 cells, the HC PL APO 40x/1,30 OIL CS2 oil immersion objective was used

After 48 h, cells were passaged 1:2 into fresh media containing 3 g ml 1 puromycin (Gibco, A1113803)