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When assessing drug efficacy for the treatment of glioblastoma multiforme (GBM), currently, the most reliable models are generated through direct injection of patient-derived cells or more frequently the isolation of glioblastoma cells endowed with stem-like features and orthotopically injecting these cells into the cerebrum of immunodeficient mice

Planta Medica 83 (5), 468475

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The lysates were incubated on ice for 30 min, followed by centrifugation at 12,000 g for 15 min at 4 C