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After the blood collection, mice were flushed with ice-cold PBS to remove residual blood

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ii) Ki67 (Cat# M724029, clone MIB1, Agilent, Santa Clara, CA, USA) to evaluate proliferation and iii) pan-cytokeratin (Cat# M082102, clone MNF116, Agilent, Santa Clara, CA, USA) as a guide for a training tumor stroma classifier
