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In regions such as the AsiaPacific, where shellfish consumption is high, shellfish allergy is not only more prevalent and clinically significant, but symptoms may also develop at an earlier age compared to Western countries [9, 10]

The thrombin cleaved protein was purified by size-exclusion chromatography on a superdex-200 10/300 increase column (GE Life Sciences) in 4 mM decyl d-maltoside (Anatrace), 0.3 mM CHS, 0.001% (w/v) 1-pamitoyl-2-oleoyl- sn -glycero-3-phospho ethanolamine (Avanti Polar Lipids), 20 mM Tris-Cl (pH 8.0), 300 mM NaCl, and 5% glycerol

15 JiaxuanL.ShufangJ.HuanL.HongyanQ.XinZ.XiaL

doi: 10.1007/s42976-021-00221-6