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10.1016/j.xcrm.2022.100763 [DOI] [PMC free article] [PubMed] [Google Scholar] 25.Slieker RC, Donnelly LA, Akalestou E et al (2023) Identification of biomarkers for glycaemic deterioration in type 2 diabetes

Blots were first incubated with 5% BSA and 0.1% Tween in Tris-buffered saline (TBS) for 1 h at room temperature, and then with either polyclonal rabbit primary antibody against Orai1 (1:1,000, rabbit Sigma-Aldrich, O8264), STIM2 (1:2,000, rabbit Sigma-Aldrich, S8572) or monoclonal mouse actin (1:100, mouse Sigma-Aldrich, A2066) at 4C overnight