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Hormones regulate several processes that affect acne development, including: Oil production Skin cell turnover Inflammation Hair follicle function Bacterial growth on the skin When hormones become imbalanced, the oil glands can become more active, producing excess sebum that mixes with dead skin cells and clogs pores

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In the molecular docking experiments, ETS1s 3D structure was used as the receptor, and the GPX4 promoter sequence was modeled as the ligand

Immunoprecipitation and western blotting Cells were lysed with RIPA buffer, and 500 g whole cell lysate was precipitated with 2 g agarose-conjugated antibody against Flag (Flag beads) and normal IgG (as a negative control)