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The sections were then washed three times in TNT buffer (0.1 M TrisHCl, 0.15 M NaCl, 0.05% Tween20, pH 7.5) for 5 min, and treated with secondary antibodies
So we take pre-op numbers on their uninvolved side and at least use that as a gauge
TABLE 1 PCR primer sequences used in the experiments
Some GP surgeries might argue that changing from B12 injections to oral B12 is not a major change
