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Exploiting these characteristics, Ec TyrRS and Ec tRNA Tyr CUA were employed as an orthogonal pair for amber suppression in yeast and mammalian cells ( Leu CUA , facilitating the effective incorporation of UAAs in yeast ( 3.2.3 Rationally engineering tRNA for improving efficiency and orthogonality Going beyond the mere adoption of tRNAs from diverse species, researchers have sought to introduce mutations into tRNAs to enhance their expression levels, stability, orthogonality, suppression capability, and interaction with binding partners such as AARS or EF-Tu

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Nielsen, L

doi: 10.1007/s11914-015-0292-x