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Quantitative Real-time PCR RNA extraction and quantitative real-time PCR were performed according to our previously reported method [27]

If any issues exist with the acquired AoPC to quantify MR by the LVSV AoPC method, an alternative approach is to use the pulmonary artery flow (PaPC), as long as no intracardiac shunts exist (LVSV PaPC)

Under such circumstances (with a TMLHE deficit) a supplementation in L-carnitine seemed efficient

Dysbiosis is associated with intestinal inflammation and reduced integrity of the gut barrier, which in turn increases circulating levels of bacterial structural components such as LPS and microbial metabolites TMAO that may facilitate the development of CVD (Novakovic et al