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After four washes for 10 min in Triton X-100- and albumin-supplemented PBS, and one wash in PBS at RT, diamidine-2-phenylindole-dihydrochloride (DAPI, 236276, Roche Diagnostics) was used as fluorescent nuclear stain at 0.1 g/mL in PBS for 10 min
Previous studies have shown that during invasion, Cryptosporidium sporozoites export proteins, such as the ROP1 and MEDLE protein families, into the cytosol of infected host cell 13,14
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