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Requirement of lectin domain in efficient enzyme reaction To investigate the functions of the GnT-IVa lectin domain in more detail, we purified soluble wild-type enzyme and its D445A mutant (Fig
doi: 10.1371/journal.pone.0008590
Therefore, we asked whether we could rescue glutamine dependency of KPK cells by forcing the export of cystine and the import of glutamate by modulating the concentration of these two amino acids in the culture media
