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Briefly, yeast cells were spheroplasted with 500 g ml 1 Zymolyase 100T, homogenized in polyvinylpyrrolidone-buffer (8% polyvinylpyrrolidone, 20 mM potassium phosphate buffer, pH 6.5, 0.75 mM MgCl 2 , and protease and phosphatase inhibitor cocktails) with a Dounce homogenizer

PLoS ONE 16:e0255956

Pillitteri attended Cornell University with a double major in mathematics and biology in 2003

Additionally, some MELAS patients experience loss of fixation sensitivity (FOS), an EEG phenomenon in which epileptic discharges are triggered by loss of central vision or gaze, characterized by idiopathic or generalized epileptic discharges following eye closure for more than 3 s (30)