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PGL-3 tethering experiments were imaged using a 3i-Zeiss spinning disk confocal microscope that consisted of a 3i spinning disk system [CSU-X1 M1 Spinning disk confocal, a Prime BSI CMOS camera and 405, 488, and 561 nm lasers (Intelligent Imaging Innovations Inc., Denver, CO)] mounted on an AxioObserver (ZEISS, Oberkochen, Germany)

Authors also thank Elisabeth Bascuana, Jordi Hernndez, Montserrat Jimnez, Damir Garcia-Cehic, Leonardo Nieto-Aponte, and Josep Gregori for their help in specimen processing

In terms of phase behavior, different fatty acid compositions contribute to various phase transitions, including gel-to-fluid, hexagonal, and liquid phases

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