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2 Materials and methods 2.1 Experimental mouse models In the acute SCI model, adult male C57BL/6N mice (1012 weeks, male, Nihon SLC, Shizuoka, Japan) were subjected to contusion injury at T9T10 using an Infinite Horizon Impactor (60 kdyn) or sham-operated by laminectomy only, as described previously (Figure 1A) ( ttw / ttw mice (1224 weeks, male, Central Institute for Experimental Animals, Kawasaki, Japan) were used, with age-matched male Institute of Cancer Research (ICR) mice as controls

The IIEF-EF improvement in this study was 5.5 points and favorably compared to 2 points for testosterone replacement, 4 points for shockwave therapy, and 4 to 8 points for PDE5 inhibitors [45]

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Measurement of AMP H9c2 cells were treated with 5 mM 2-AB for 30 min