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The method is not only time-consuming but also limits the identification/quantification by DIA to the peptides identified by DDA, which hinders the inherent advantages of DIA of unbiased measurement

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Such process improvements are especially relevant for disulfide bond-containing therapeutics, such as bispecific antibodies (10) Summary Comprehensive and quantitative metabolic analysis can guide actionable strategies for optimizing bioprocesses, provided the data are promptly delivered

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