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Description
Expression and purification of Authentic M pro and C300S M pro The SARS-CoV2 M pro -encoding sequence and C300S mutant sequence were cloned into pGEX-4T1 vector (Genscript) with N-terminal self-cleavage site (SAVLQ/SGFRK) and C-terminal His 6 -tag as previously designed by others 6
This is antagonised by cellular systems which include naturally occurring enzymes e.g
However, their functions and thermoregulatory mechanisms necessitate further investigation
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