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Alkylation is performed at low pH (5.0), where RSSH are expected to maintain high reactivity (owing to their lower p K a and increased nucleophilicity), whereas the majority of free thiols remain protonated and less reactive (Fig
doi: 10.3390/genes15101304 66 MenoldMMShaoYWolpertCMDonnellySLRaifordKLMartinERet al
