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Description
Cell lysates or soluble fractions were stored frozen in 1 ml aliquots for further experiments as repeated freeze-thawing clearly influenced the results obtained in a protein dependent manner
cDNA synthesis was performed by reverse transcription of the RNAs using the M-MLV Reverse Transcriptase kit (Cat#M1701, Promega, WI) according to the manufacturers protocol and screened for GluR3 by PCR and sequencing
Nature 418 , 3839 (2002)
Thus, improved formulations can increase both ALA absorption and bioavailability, leading to a raise in therapeutic efficacy
