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Disulfide bonds were reduced with 10 mM dithiothreitol for 30 min at RT before adding 25 mM iodoacetamide and incubating for another 30 min at RT in the dark

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You might consider vitamin B12 injections, B12 Shots if you are: Following a vegan or vegetarian diet that may not provide sufficient vitamin B12
R., Cohen, D
