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Reason 3: The preservative stops working as designed Even if benzyl alcohol is still technically present after a freeze/thaw cycle, its antimicrobial effectiveness can degrade
wherein the authors successfully demonstrated that CRISPR/Cas9-assisted genome editing can knock-in Cre and silent blue fluorescent protein (BFP) expression, thereby enabling precise monitoring of the differentiation process [87]

They found that iron, ACSL4, and MDA levels increased with increasing reperfusion time, with concomitant decreases in GPX4 levels, and deferoxamine treatment significantly ameliorated myocardial injury [88]

doi: 10.1038/nrc727