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For experiments in 384-well plates, wells were aspirated, rinsed with PBS and incubated with a fixative and staining solution containing 3.7% formaldehyde (Fisher Scientific, F75P-1GAL) and 5 g ml 1 Hoechst 33342, trihydrochloride, trihydrate (Invitrogen, H1399)

These groups received intravenous treatments of oridonin, orid-liposome, orid-liposome-MAL, and TLR2 pep-orid-liposome at a dosage of 5 mg/kg (equivalent to the active ingredient of oridonin), with PBS serving as the control, administered every other day

Each lot undergoes analytical verification and quality-control review

In 2002, buprenorphine (Subutex/Suboxone) was a game-changer: as a partial agonist, it offered a safer, more flexible option that could be prescribed in outpatient settings and filled at the local pharmacy