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Description
Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

Chemical Specifications

In that context, NAC may play a supportive role in helping the body maintain balance and resilience when the pollen flies

Glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) activities were enhanced
