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Semen Quality Analysis Sperm function parameters such as sperm motility, sperm count, and morphological abnormalities, following the WHO guidelines ( Testicular Cholesterol and Sex Hormone Analyses Frozen testis tissues were thawed, homogenized in an ice-cold radio immunoprecipitation assay (RIPA) lysis buffer (Thermo Fisher Scientific, Waltham, MA, USA), protease inhibitors and phosphatase inhibitors mixture, centrifuged at 14,000 g (4C) for 20 min and used the supernatant to measure the testicular concentrations of cholesterol, estradiol and testosterone with commercial ELISA kits according to the manufacturer's instructions (MBS164208

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