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Cells were plated onto 96-well plates at a final concentration of 1104 cells/well in culture medium with drugs (piroxicam, ibuprofen, indomethacin, prednisone and betamethasone) or cytokines (TNF-, IL-1, IL-6, IL-8 and IL-10)

However, since there are relatively few cells in the degenerated IVD and cell viability is impaired, stimulation of the remaining cells may be insufficient to achieve repair [13]

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Some of these factors include your skin type, tone, layering of the tattoo, location, pigmentation, the amount of ink applied to your tattoo, the kind of pigment used, whether homemade pigment or professional ink was used, and any pre-existing scars from the tattoo