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27 The aorta and major branches were fixed in 10% buffered formalin while a portion of the common carotid artery was stored in liquid nitrogen for RNA extraction or ultrasonically fragmented for supernatant extraction (Supplementary Fig

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These assays are often performed with cells derived from a nave population, where the frequency of antigen-specific cells is relatively low and precludes a clear positive result due to low signal-to-noise ratios (88)