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On day 0, the Matrigel matrix was diluted to 5 mg/mL with ice-cold SCL4.1/NG108 complete cell culture medium, and 5 L was added into each well of a pre-chilled 96-well plate, spread evenly with a pipet tip, and incubated at 37 C for 1520 min to form a gel

This damage is invisible, you cannot see it, but your body will notice reduced efficacy

Reduced waste : The 28-day post-puncture stability window means researchers can use the entire vial across a research protocol without waste