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Monitor your urine colour pale straw indicates adequate hydration, whilst dark yellow suggests insufficient fluid intake

In brief, 20 l of Viability/Cytotoxicity Reagent containing both GF-AFC was added to each well, followed by 30 sec mixing at room temperature and incubation at 37C for 30 min

doi: 10.4049/jimmunol.177.5.2810 144 Girn-GonzlezJAMoralFJElviraJGarca-GilDGuerreroFGavilnIet al
